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Journal: Frontiers in Physiology
Article Title: Different Levels of Expression of the Clock Protein PER and the Glial Marker REPO in Ensheathing and Astrocyte-Like Glia of the Distal Medulla of Drosophila Optic Lobe
doi: 10.3389/fphys.2018.00361
Figure Lengend Snippet: (A) Horizontal section of the medulla of NP6520 -Gal4/UAS-mCD8-GFP flies immunolabeled with anti-PER antibody. The nuclei of GFP-expressing EnGl (frames 1–3) are PER-positive, which is well visible in higher magnification (panels 1–3). The processes of EnGl (arrowheads in panels 1–3) span the medula neuropil (Mn). R, retina; Lc, Lamina cortex; Ln, lamina neuropil; oCh, outer chiasm; Mc, medulla cortex; iCh, inner chiasm. Scale bars: 20 μm for (A) and 10 μm for panel 1, 2, and 3. (B) The nuclei of GFP-positive EnGl show high level of REPO-specific immunofluorescence (arrow). Arrowhead-the EnGl processes. Scale bar: 10 μm. (C) The distal part of the medulla in horizontal section of NP6520 -Gal4/UAS-mCD8-GFP flies immunolabeled with anti-DmMANF antibody. The processes of EnGl are marked with arrows. R, retina; Lc, lamina cortex; Ln, lamina neuropil; Mc, medulla cortex; Mn, medulla neuropil. Scale bar: 20 μm. (D-D”) Higher magnification of EnGl marked in (C) . The GFP-positive processes of EnGl ( D , arrows) are marked with DmMANF (D',D”) . DmMANF is also visible in the perinuclear space of cell bodies in the medulla cortex (D', arrowheads). Scale bar: 10 μm.
Article Snippet: Cryosections of 20 μm thickness were cut and immunolabeled with primary antibodies as follows: mouse anti-REPO Ab (1:40, Developmental Studies Hybridoma Bank), mouse anti-PDF Ab (1:500, Developmental Studies Hybridoma Bank) rabbit anti-GFP Ab (1:1000, Novus Biologicals), mouse anti-GFP Ab (1:1000, Novus Biologicals), rabbit anti-PER Ab (1:1000, a kind gift of R. Stanewsky), and rabbit anti- Drosophila melanogaster MESENCEPHALIC ASTROCYTE-DERIVED NEUROTROPHIC FACTOR or
Techniques: Immunolabeling, Expressing, Immunofluorescence